The laboratory zebrafish (Danio rerio) is a well-established vertebrate animal model in frontline areas of modern biology research, including screening of therapeutic molecules and assessment of toxic chemicals. High fecundity is one of the important reproductive traits in zebrafish females that benefit researchers in obtaining a large number of embryos for high throughput experiments. External fertilization offers easy access to the embryos up to 5 days post fertilization without ethical concerns whilst optical transparency of developing embryos allows for imaging of the entire embryo, which is not practical in mammalian models. Despite being prolific breeders, laboratory zebrafish exhibit a reduction in reproductive performance aftertwo years. In vitro fertilization, which uses frozen sperm, is an effective method for reviving genetically modified fish lines. In this study, we attempted to us e an IVF approach to re store zebrafish lines from aged zebrafish. A total of 12 zebrafish lines were reproduced using the IVF procedure, including aged fish that are unable to spawn naturally. A total of 974 (66.96%) and 1438 (55.67%) embryos survived utilizing the IVF procedure with germ cells from both mutant and wild-type parents, respectively. Zebrafish embryos survived at a rate of 67.34% in the 2–3-years age groupand55.48% in the 3-4 years age group.

Eight-week-old male and female BALB/c mice that were procured and kept for quarantine experienced 10% mortality in males and 3.3% in females from the second day onwards with partially mutilated carcasses in both cages. Considering the stress due to travel Vitamin C was provided in the drinking water from day 4. The percentage of mortality was reduced as compared to the first day however mortality continued to 5th day. Furthermore, the application of eucalyptus oil in the cage and animal body surface of the animals for one week repeatedly results in a substantial decrease in % mortality in male and female mice.

Laboratory Animal Sciences play a pivotal role in scientific research, significantly contributing to various fields such as medicine, biology, and toxicology. This discipline is crucial in advancing scientific knowledge, improving healthcare outcomes, and ensuring animals’ ethical and responsible use in research. Animal health is paramount in this field, yet it is sometimes neglected during experimentation. To address this concern, many countries have developed their regulations for animal welfare. Moreover, globally, the WOAH (World Organisation for Animal Health) has introduced guidelines and regulations mandating research organizations to prioritize animal health. An international organization is ICLAS (International Council for Laboratory Animal Science) which is dedicated to advancing laboratory animal science worldwide, promoting the ethical use of animals in scientific research, and ensuring their welfare. In conjunction with ICLAS, several other organizations share responsibilities for promoting good animal practices globally. This paper provides a comprehensive overview of the Laboratory Animal Sciences within the GCC countries. A brief infrastructure detail that supports animal welfare and the significance of these facilities in facilitating cutting-edge research is explored, alongside an analysis of the accreditation and management protocols crucial for their effective op - eration. Additionally, the paper scrutinizes the current laws and regulations governing laboratory animal welfare and experimentation in the GCC region. As a forward-looking contribution, the paper outlines prospective developments and expansion opportunities for laboratory animal sciences in the GCC, aiming to propel scientific advancements in the region. This research provides a valuable resource for scientists, policymakers, and stakeholders invested in promoting ethical and effective laboratory animal research practices in the Gulf region.

The rising incidence of obesity poses a major global health issue, shaped by a complex interaction of genetic, environmental, and behavioral elements. This review focuses on the important function of various animal models in the study of obesity, emphasizing their contributions to comprehending the underlying mechanisms of this intricate condition. Rodent models, especially genetically modified strains such as ob/ob and db/db mice, have revealed crucial insights into the hormonal and genetic pathways that govern energy balance. Furthermore, models of diet-induced obesity and new organisms like zebrafish and C. elegans present innovative methods for investigating the impacts of high-fat diets and genetic differences. The review also ad dresses the limitations of existing animal models, including their in ability to completely mimic the human experience of obesity due to gene-environment interactions and varying metabolic responses. By integrating findings from a range of animal models, this research seeks to deepen the understanding of the causes of obesity and aid in formulating specific therapeutic approaches, ultimately tackling the escalating obesity crisis.

Lactation induces physiological changes in mammals, which can influence hematological and biochemical parameters. This study aims to compare the hematological and biochemical profiles of lactating and non-lactating Rhesus monkeys (Macaca mulatta) to better understand the impact of lactation on their metabolic and physiological status. The aims of this study were to verify if significant changes occur in hematological and biochemical parameters in Rhesus Macaques during Lactation period and Post lactation Hematological and biochemical parameters were evaluated in 12 Female Rhesus Macaques that were Lactating and Post Lactation .On Hematology analysis we could find the significant changes in neutrophil and Lymphocytes count between the two groups . Biochemistry analysis showed significant changes in Triglycerides level . Our findings provide reference data for researchers and contribute to a better understanding of primate biology in different reproductive states